FastQCFastQC Report
Mon 11 Dec 2017
frozen-129-F-ATAC-2-182_GAGGGGTT_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefrozen-129-F-ATAC-2-182_GAGGGGTT_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18400379
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT26000.024.23135120
CGTCTAG22900.022.2741831
TAGGACG165050.021.5967924
TCGCCGG69100.020.579365120
GGACGTG170150.020.028566
AGGACGT186100.019.5392555
TCTCGGG53550.019.048077120
GTCCTAC188650.018.6405681
CGTATCC135450.018.427906120
GACGTGG98550.017.837837
GTAGGAC185050.017.6412013
CCGTGAT105300.017.43645927
CGTGATT107950.017.3975528
TTGACGG27300.017.36324331
GACGTGA101650.016.8216467
CGGTGGG64800.016.75966120
TACAGTG237300.016.7394815
CTACGGT18200.016.48247713
CTACAGT226100.016.4554394
ATATGGC332900.016.31022315
Produced by FastQC (version 0.11.2)