FastQCFastQC Report
Mon 11 Dec 2017
frozen-129-F-ATAC-2-182_GAGGGGTT_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefrozen-129-F-ATAC-2-182_GAGGGGTT_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18400379
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCTCGTATGCCGT746330.40560577583755203No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG21050.029.923941
TAGGACG176200.022.3018344
CTACGGT19550.021.78489513
GGACGTG179900.021.5752546
TACGGTG20600.020.67449814
GTCCTAC178400.020.5460321
AGGACGT200800.020.316465
TTGACGG27900.019.1352631
GTAGGAC196950.018.5206033
CTAGACG25200.018.331493
GACGTGA110700.018.0996347
TACAGTG228500.018.0898885
TCCGCAA23300.018.0214933
TAGACGC36850.017.9085484
TATGGCG201900.017.58980816
TGGCGAG198100.017.56397418
CTACAGT216300.017.362934
ATATGGC358700.017.208615
CCTACAG214850.017.0892753
CGGCTAT32400.017.03328735
Produced by FastQC (version 0.11.2)