FastQCFastQC Report
Mon 11 Dec 2017
frozen-129-F-ATAC-2-182_GAGGGGTT_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefrozen-129-F-ATAC-2-182_GAGGGGTT_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18391880
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG22000.025.9123941
CGCCGTT27000.021.556368120
TAGGACG171750.021.312764
TCTCGGG53700.020.782907120
GTCCTAC191100.020.0967521
GGACGTG177650.020.0950326
TCGCCGG71250.019.537579120
CGTATCC141450.019.258413120
AGGACGT195800.019.2442255
CCGACGG36950.018.999363120
GGTACGA19100.018.5332718
CGGTGGG65250.018.391499120
GTAGGAC187950.018.3902133
TACAGTG242200.018.3072765
TTGACGG27750.018.16329231
CTACAGT231400.017.9971074
GACGTGA106650.017.8895117
AGTGGAC151150.017.584418
CCGTGAT106900.017.56896227
CGTGATT111000.017.19030628
Produced by FastQC (version 0.11.2)