FastQCFastQC Report
Mon 11 Dec 2017
frozen-129-F-ATAC-2-182_GAGGGGTT_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefrozen-129-F-ATAC-2-182_GAGGGGTT_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18391880
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCTCGTATGCCGT734460.39933927363597416No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG21150.024.1091961
TAGGACG175300.022.5197814
GTCCTAC184650.021.4746591
GGACGTG185200.020.8937556
AGGACGT202650.020.3981345
GTAGGAC200800.019.1520183
TACAGTG247750.018.1378575
CTACGGT17900.018.09631213
CTACAGT228550.017.929154
AGTGGAC154500.017.784128
GACGTGG112750.017.7183447
CCTACAG221500.017.7143083
TTGACGA106200.017.62303260
CGACTTG97550.017.49168464
TTCCTCG127950.017.4889143
GACGACT100300.017.46354562
TTGACGG26900.017.39401831
ACGTGGA117650.017.3373378
TGTAGGA258400.017.295882
TCCTCGC121050.017.24680544
Produced by FastQC (version 0.11.2)