FastQCFastQC Report
Mon 11 Dec 2017
frozen-129-F-ATAC-2-182_GAGGGGTT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefrozen-129-F-ATAC-2-182_GAGGGGTT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18401832
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG23800.024.2048281
CGCCGTT26250.022.856699120
TAGGACG169600.021.9368154
GGACGTG176550.020.2889736
TCTCGGG55850.020.196566120
GTCCTAC188800.020.182711
AGGACGT195850.019.7303455
TCGCCGG63250.019.635983120
TTGACGG25900.019.23296731
CCGACGG38700.019.069399120
CGGTGGG66800.018.772093120
GTAGGAC188950.018.2611753
CGTATCC142600.018.05014120
GACGTGA106450.017.9799737
CTACAGT228650.017.7149644
CCGTGAT110150.017.65340427
TACAGTG240100.017.6185155
TGCTCGA24200.017.60234310
CGTGATT110200.017.59093328
AGTGGAC148100.017.4197088
Produced by FastQC (version 0.11.2)