FastQCFastQC Report
Mon 11 Dec 2017
frozen-129-F-ATAC-2-182_GAGGGGTT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefrozen-129-F-ATAC-2-182_GAGGGGTT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18401832
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCTCGTATGCCGT734580.39918851557823154No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG21850.029.9262221
TAGGACG180950.022.3798624
TTGACGG25950.021.72943531
GGACGTG186350.020.9567956
GTCCTAC185950.020.1632561
AGGACGT207600.019.7379515
CGGCTAT33400.019.21784435
GTAGGAC196500.018.8074683
TACAGTG236250.017.5474855
GGTACGA16450.017.50474218
CGACTTG101250.017.45324164
CTACAGT220750.017.393684
AGTGGAC151850.017.2246238
GACGTGG114900.017.177267
TTGACGA108400.017.15419260
GACGTGA110450.017.1089297
ATATGGC355300.016.95030615
TGGCGAG194700.016.94638418
TGACGGC33150.016.82896432
TATGGCG193900.016.79813616
Produced by FastQC (version 0.11.2)