FastQCFastQC Report
Mon 11 Dec 2017
frozen-129-F-ATAC-2-182_GAGGGGTT_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefrozen-129-F-ATAC-2-182_GAGGGGTT_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18342569
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT25950.022.19521120
TAGGACG168950.022.091074
TCGCCGG63350.022.06656120
TCTCGGG52550.021.00731120
GGACGTG174850.020.7275316
GTCCTAC188900.020.1397881
AGGACGT193650.019.4902115
CGTCTAG20650.018.8882141
CGTATCC138000.018.824966120
CCGACGG36100.018.613848120
GACGTGG103200.017.9077177
TACAGTG239200.017.6350825
GTAGGAC189550.017.443173
CGACTTG93850.017.38865364
CTACAGT225750.017.3568134
AGTGGAC152550.017.108318
GACGTGA107900.016.9608487
TGGCGAG193600.016.85891218
CCTACAG220800.016.8496253
CGGTGGG65600.016.828266120
Produced by FastQC (version 0.11.2)