FastQCFastQC Report
Mon 11 Dec 2017
frozen-129-F-ATAC-2-182_GAGGGGTT_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefrozen-129-F-ATAC-2-182_GAGGGGTT_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18342569
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCTCGTATGCCGT730450.39822666061662354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG21700.032.070631
TTGACGG25800.023.48219531
TAGGACG178300.022.4772224
GTCCTAC184450.021.7273621
GGACGTG181250.021.580776
GTAGGAC196700.020.9541423
AGGACGT199900.020.3503685
TGACGGC32700.019.811332
CGGCTAT33650.019.25199335
GGTACGA17500.019.1960518
GACGTGG107950.018.561297
TACAGTG236400.018.350435
GACGGCT33350.018.34599733
CTACGGT19200.018.12092413
CCGTGAT107000.018.10733427
CGTGATT109500.017.91304628
CCTACAG224600.017.710113
CTACAGT226050.017.4903454
TCCTACA249600.017.3783632
AGTGGAC152950.017.3755518
Produced by FastQC (version 0.11.2)