FastQCFastQC Report
Mon 11 Dec 2017
frozen-129-F-ATAC-2-182_GAGGGGTT_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefrozen-129-F-ATAC-2-182_GAGGGGTT_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18337122
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT25650.022.921942120
CGTCTAG22900.022.5379411
TAGGACG170950.021.9409314
TCTCGGG53350.021.141516120
GGACGTG173450.020.6543256
GTCCTAC193050.020.5486321
AGGACGT193300.019.5592785
TCGCCGG67250.019.448086120
CCGACGG34700.019.364275120
GACGTGG101450.018.9261687
CGTATCC147200.018.66682120
GTAGGAC192150.018.4273513
TACAGTG245900.018.1087935
CGGTGGG66700.017.89946120
AGTGGAC156650.017.6569798
TTCCTCG129600.017.35942843
CTACAGT234550.017.2708024
CCTACAG229800.017.2102283
GGACATT168900.017.15660511
TTGACGG25350.017.04618331
Produced by FastQC (version 0.11.2)