FastQCFastQC Report
Mon 11 Dec 2017
frozen-129-F-ATAC-2-182_GAGGGGTT_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefrozen-129-F-ATAC-2-182_GAGGGGTT_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18337122
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCTCGTATGCCGT726170.396010889822296No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG23250.028.6424161
TTGACGG25750.023.76127831
CTACGGT17900.023.1226213
TAGGACG178200.022.6911814
GGACGTG180750.021.6738596
TACGGTG18200.021.42447314
CGGCTAT31800.020.56103935
AGGACGT202450.020.2399315
GTAGGAC199200.019.7270283
GTCCTAC181500.019.336991
GACGGCT31150.018.29410733
GACGTGA109950.018.1137627
GACGTGG110450.017.9231387
TGACGGC34150.017.91663232
ACGGCTA31900.017.67634
TACAGTG241050.017.6459545
TAGACGC37400.017.6451764
ACGTGGA113100.017.5031878
GGTACGA18200.017.46918518
ATATGGC366550.017.18399815
Produced by FastQC (version 0.11.2)