FastQCFastQC Report
Mon 11 Dec 2017
frozen-129-F-ATAC-2-182_GAGGGGTT_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefrozen-129-F-ATAC-2-182_GAGGGGTT_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18301953
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG167800.022.354654
CGTCTAG23900.022.350631
CTACGGT17950.021.39137613
TCGCCGG65550.021.141932120
GGACGTG169900.020.7681276
CGCCGTT28900.020.343872120
GTCCTAC186850.019.9478511
AGGACGT189700.019.7103615
TACGGTG19050.019.52773514
CCGACGG34300.019.239948120
GTAGGAC188200.018.9755763
AGTGGAC147150.018.4709668
TCTCGGG49250.018.393995120
CTAGACG23800.018.157383
CGTATCC153300.018.119417120
TACAGTG243250.017.9619065
GACGTGG95700.017.8062427
TTGACGG26650.017.56507131
CTACAGT233200.017.5523644
GACGTGA107000.017.4959167
Produced by FastQC (version 0.11.2)