FastQCFastQC Report
Mon 11 Dec 2017
frozen-129-F-ATAC-2-182_GAGGGGTT_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefrozen-129-F-ATAC-2-182_GAGGGGTT_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18301953
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCTCGTATGCCGT727670.39759144830062676No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG20450.032.5602151
TAGGACG173950.024.1394634
TTGACGG25900.022.6960731
GGACGTG180950.022.209416
TACGGTG21300.021.12145414
AGGACGT200750.020.916865
GTCCTAC181400.020.8003641
GTAGGAC191800.020.11023
CTACGGT21900.019.99470513
ACGGCTA28950.019.6834234
CGGCTAT32050.019.65109635
GACGTGG109400.018.9156047
TCCGCAA23250.018.57521833
GACGTGA112450.018.4558937
TAGACGC36750.018.281624
CGACTTG98350.018.27674164
ACGTGGA112000.018.2091648
CTAGACG26050.018.1917043
TACAGTG237700.018.0691555
CTACAGT221400.017.9634574
Produced by FastQC (version 0.11.2)