FastQCFastQC Report
Mon 11 Dec 2017
frozen-129-F-ATAC-2-182_GAGGGGTT_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefrozen-129-F-ATAC-2-182_GAGGGGTT_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18285952
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG21400.025.2385831
CGCCGTT25900.021.774189120
TAGGACG170100.021.027044
GTCCTAC179900.020.9824261
TCTCGGG52000.020.882818120
CTACGGT18850.019.7335913
CGTATCC155000.019.585413120
GGACGTG172000.019.5013626
TCGCCGG65250.019.400629120
GTAGGAC190350.019.010813
AGGACGT194100.018.241595
CTACAGT226900.017.9850084
TACAGTG242850.017.7181055
CGACTTG90600.017.6156864
GGTACGA14650.017.61104418
AGTGGAC149800.017.502128
CCTACAG216800.017.2173923
TCCTACA239200.017.110372
GACGTGG99150.017.0647967
TACGGTG21150.017.02069714
Produced by FastQC (version 0.11.2)