FastQCFastQC Report
Mon 11 Dec 2017
frozen-129-F-ATAC-2-182_GAGGGGTT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefrozen-129-F-ATAC-2-182_GAGGGGTT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18285952
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCTCGTATGCCGT728070.39815810519463246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG22750.027.6887041
GTCCTAC183700.021.292821
TAGGACG172950.021.262394
TTGACGG25600.020.15241431
GGACGTG179000.019.8047146
GTAGGAC191900.019.631653
AGGACGT197000.018.7275035
GGTACGA17150.017.83843818
CTACAGT224050.017.805284
TACAGTG237950.017.7735585
TTCCTCG123950.017.71514543
AGTGGAC150150.017.5783028
CCTACAG221250.017.488343
TCCTACA247700.017.4866222
TCCTCGC115750.017.31134844
GACGTGG108850.017.1933427
TGGCGAG200100.017.1174818
TATGGCG205000.017.08910416
GACGACT98000.017.07763562
CGACTTG95250.017.03048364
Produced by FastQC (version 0.11.2)