FastQCFastQC Report
Mon 11 Dec 2017
frozen-129-F-ATAC-2-182_GAGGGGTT_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefrozen-129-F-ATAC-2-182_GAGGGGTT_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18326505
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT23800.060.8841194
CGACGTA31350.042.96639692
TCGACGT33050.041.16800791
CACCGTA35900.040.5527893
TGTCGGT24300.039.7542393
GTGATCG20800.038.92108591
TACGAGT19250.038.16755794
ACGCCGT25450.037.15594594
ATCGCCG20650.036.56825394
GGTCGAC37950.036.21170889
TAGATCG27500.035.87266588
TGATCGC22550.035.59891592
ACGTATC36750.034.80180794
GTCGATC24900.034.42488592
TCACCGT44650.034.281792
TGGTCGA41050.034.14148788
GTCACCG44850.034.12882691
GTCGACG40600.033.6799490
GATCGCG28900.033.19117490
CCGTCGA18900.033.11519691
Produced by FastQC (version 0.11.2)