FastQCFastQC Report
Mon 11 Dec 2017
frozen-129-F-ATAC-2-182_GAGGGGTT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefrozen-129-F-ATAC-2-182_GAGGGGTT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18326505
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCTCGTATGCCGT710900.3879081145041021No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACG4500.024.00086499
TAGGACG175650.023.703144
CGTCTAG23050.022.1228681
GGACGTG182450.022.0614556
GTCCTAC181050.021.7369561
GTAGGAC195750.021.1158493
AGGACGT203100.020.9721535
CTACGGT18800.020.74055913
TACGGTG20250.019.84758414
TAGACGC37600.019.62514
GACGTGG116600.019.1899077
CGACTTG102900.019.07956164
GACGACT105200.018.87390962
TTGACGA109100.018.8590160
CTACAGT221300.018.623874
ACGTGGA119550.018.6159238
TGACGAC109750.018.31006261
CTGTAGG232500.018.2427331
CGTGGAA119050.018.2406129
TACAGTG241150.018.1357235
Produced by FastQC (version 0.11.2)