FastQCFastQC Report
Mon 11 Dec 2017
WSB-M-ATAC-2-17_TGCTGGGT_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-M-ATAC-2-17_TGCTGGGT_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16371915
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG56050.020.125055120
CGGTGGG70850.018.969812120
CGTATCC128750.018.547728120
TAGGACG65150.017.961894
TCGCCGG85700.016.032806120
CGCCGTT31300.015.143897120
CGTCTAG17850.015.1288571
TAGATCC149100.014.808977120
GACGAGG82850.014.628969120
AGGACGT90300.014.4862775
GACCGTT22800.014.4728327
GGACGTG79550.014.4052746
GTCCTAC100200.014.31403351
GTAGGAC99550.014.1663653
TGACGCC56950.013.801695120
GCGTAAA18100.013.593681
TGCGATA18250.013.47886646
AGACCGT27000.013.1104196
CCGACGG51400.013.074039120
TAGTCGA18000.013.00110155
Produced by FastQC (version 0.11.2)