FastQCFastQC Report
Mon 11 Dec 2017
WSB-M-ATAC-2-17_TGCTGGGT_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-M-ATAC-2-17_TGCTGGGT_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16371915
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG20050.022.7389011
TAGGACG70850.018.2900414
GTCCTAC96700.017.6182231
TTGACGG24300.016.29221231
CGTAATC17400.016.20342822
CTAGACG19850.015.7161043
TAGACGC32150.015.3014874
GTAGGAC104200.015.142243
CGTACGT17650.014.6139934
TACACCG23100.014.5436745
GGACGTG87550.014.5266196
AGGACGT98900.014.497725
CTACGGT19150.014.40874413
CCGTACG17800.014.15375533
CTACACG24600.014.1446974
CGGCTAT30750.013.85031435
TAGTCGA16550.013.7747495
TACGGTG21350.013.76687314
TAACCGC23300.013.6459826
TTGGACG23800.013.61107317
Produced by FastQC (version 0.11.2)