FastQCFastQC Report
Mon 11 Dec 2017
WSB-M-ATAC-2-17_TGCTGGGT_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-M-ATAC-2-17_TGCTGGGT_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16370038
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG59150.020.997776120
CGTATCC134200.020.477203120
TCGCCGG90450.019.569113120
TAGGACG65600.018.203664
GTCCTAC101800.017.9198861
GCGTAAA18600.016.4538171
TACGCGT23050.016.1390195
ACGCGTA23150.016.068526
CGGTGGG72450.016.06649120
CGCGTAT23200.016.0337417
TACACCG24500.015.9185545
CGTCTAG16750.015.7633061
GACGAGG88050.015.74129120
ATACGCG24350.015.2792544
TAGATCC158900.014.650941120
TACAGTG159800.014.3806465
ACTGCGA38200.014.2924898
CCTACAG138650.014.2824753
GGACGTG81500.014.2816786
CTACAGT144300.014.222284
Produced by FastQC (version 0.11.2)