FastQCFastQC Report
Mon 11 Dec 2017
WSB-M-ATAC-2-17_TGCTGGGT_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-M-ATAC-2-17_TGCTGGGT_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16370038
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG19300.023.3118461
TAGGACG73900.019.9722544
TACGCGT22300.017.2190385
GTCCTAC98050.016.7027421
CTAGACG17500.016.456583
GTAGGAC108200.016.4134863
ATACGCG22550.016.2300434
ACGCGTA24100.015.68310456
GGACGTG88550.015.3796276
AGGACGT101500.015.0732845
CGACTTG45750.014.97185164
GTAGCGT18750.014.71756424
TTGACGG26500.014.48719331
TTATACG19150.014.4100582
TAGACGC30200.014.3041474
TTGACGA47250.014.21865260
TATACGC18700.014.1171653
AATGACG51100.014.11009974
TACGCCG17950.014.0384315
CGGCTAT31700.013.81407335
Produced by FastQC (version 0.11.2)