FastQCFastQC Report
Mon 11 Dec 2017
WSB-M-ATAC-2-17_TGCTGGGT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-M-ATAC-2-17_TGCTGGGT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16351862
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG58550.021.519901120
CGTATCC131150.018.482508120
TAGGACG68700.017.819454
CGTCTAG19900.017.1886841
CGCCGTT29600.016.013388120
CGGTGGG69700.015.408773120
TCGCCGG86250.015.304227120
GTCCTAC98950.014.9190181
GACGAGG87150.014.388871120
GGACGTG81550.014.2736026
TAGATCC159000.014.226304120
GTAGGAC101250.014.22450353
CCGACGG50650.014.21509120
AGGACGT95550.014.1296555
GACCGTT24050.013.9706567
AGACCGT31150.013.8685256
TGACGCC58450.013.652588120
GTCCTAA90350.013.6159071
GCGAATT22300.013.45289119
TACACCG26900.013.1607145
Produced by FastQC (version 0.11.2)