FastQCFastQC Report
Mon 11 Dec 2017
WSB-M-ATAC-2-17_TGCTGGGT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-M-ATAC-2-17_TGCTGGGT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16351862
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG16800.021.0677031
TAGGACG70000.018.1704374
CTACGGT16800.016.78105413
GTCCTAC96750.016.3691981
CCGTACG17700.015.92846133
CGTACGT17000.015.87905834
TAGACGC31350.015.3101694
CTAGACG20100.015.223053
CGTCTAA18200.015.1621521
TACGGTG18000.014.99578914
TACACCG24250.014.844555
GGACGTG82000.014.7781466
CGTAATC18700.014.75801122
GTGTACG25400.014.40691
AGGACGT95150.014.3134595
GTAGGAC103850.014.2698033
TAGTCGA15650.014.184535
CGTACAC25000.013.9192413
TACACTG146400.013.8926855
TATTACG14700.013.8758182
Produced by FastQC (version 0.11.2)