FastQCFastQC Report
Mon 11 Dec 2017
WSB-M-ATAC-2-17_TGCTGGGT_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-M-ATAC-2-17_TGCTGGGT_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16327316
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG19400.021.6522791
TCTCGGG56550.019.733547120
CGTATCC133550.018.643566120
TAGGACG70350.018.3382424
CGGTGGG70100.016.175907120
GTCCTAC99150.015.79628851
TCGCCGG84900.015.4760895120
GACGAGG84500.014.910336120
AGGACGT93900.014.5697675
GGACGTG85400.014.5440896
TTGACGG26000.014.54185231
GTGTACG24750.014.3048751
TAGATCC164150.014.254387120
GCGTAAT15250.013.7722691
CCGACGG49300.013.386609120
GTAGGAC106650.013.3350553
CGCCGTT30650.013.115026120
TGACGCC56650.012.8147545120
GTACCGT10350.012.75429256
TAATCGA16250.012.55412121
Produced by FastQC (version 0.11.2)