FastQCFastQC Report
Mon 11 Dec 2017
WSB-M-ATAC-2-17_TGCTGGGT_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-M-ATAC-2-17_TGCTGGGT_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16327316
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG18650.020.9097121
TAGGACG68450.017.7929024
CGTAATC17250.015.99684722
GTCCTAC98250.015.7543561
GTAGGAC105300.015.6685393
GCGTAAA17550.015.3832791
CGTACGT17200.015.34473134
TAGACGC33150.015.0216874
CCGTAAC20650.014.81439329
CTAGACG19350.014.5727353
GCGAATT21450.014.54294419
AGACCGT28900.014.5308976
TTGACGG25500.013.87859531
TACACCG23650.013.6998165
TACGCGT20750.013.5903885
GGACGTG85650.013.58834656
CGTCTAA17850.013.4442091
TGCGAAT23450.013.30232218
ATACGCG21200.013.3010584
AGGACGT95300.013.284395
Produced by FastQC (version 0.11.2)