FastQCFastQC Report
Mon 11 Dec 2017
WSB-M-ATAC-2-17_TGCTGGGT_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-M-ATAC-2-17_TGCTGGGT_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16285621
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG69250.019.3254154
TCTCGGG55650.018.758226120
CGTATCC133550.018.732677120
CGTCTAG18500.017.1930071
GACGAGG81600.016.836546120
CGGTGGG73250.016.462511120
GTCCTAC102350.015.8901931
CGCCGTT31150.015.600362120
GGACGTG83100.015.4534636
TCGCCGG87350.015.384833120
TAGATCC166800.014.962528120
CGACTTG46200.014.93512664
AGGACGT96100.014.8626865
GTAGGAC103450.014.6770153
CCGACGG50250.014.565703120
TACGCGT20800.014.4261485
CTACGGT18450.014.30847413
GTATACG6306.348273E-1014.2888881
TTGACGA48100.014.09569260
ATACGCG21800.014.0396864
Produced by FastQC (version 0.11.2)