FastQCFastQC Report
Mon 11 Dec 2017
WSB-M-ATAC-2-17_TGCTGGGT_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-M-ATAC-2-17_TGCTGGGT_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16285621
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG19700.018.5773051
TAGGACG69900.016.9942424
GGTACGA15700.016.43003818
CTACGGT18650.016.4032213
CTAGACG19150.015.66463853
GTCCTAC98150.015.6483571
TAGACGC32750.015.02160554
GTAGGAC106050.014.82213
TACGGTG19750.014.57954214
TACGCCG18850.014.32237155
AGGACGT98800.013.9664065
CGGCTAT30600.013.91810335
CGACTTG47100.013.6491864
ACGCGTA20400.013.528126
CCGTACG17350.013.4845019
GGACGTG85600.013.4566586
GTGTAGA93150.013.3323461
TACACGG24750.013.3321825
CGTACGT16650.013.33001234
TTGACGA50050.013.30317660
Produced by FastQC (version 0.11.2)