FastQCFastQC Report
Mon 11 Dec 2017
WSB-M-ATAC-2-17_TGCTGGGT_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-M-ATAC-2-17_TGCTGGGT_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16292463
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG58850.021.815378120
TAGGACG66450.018.967284
CGTATCC141000.018.423164120
TCGCCGG85300.017.16075120
CGTCTAG18700.016.3688681
CGCCGTT31600.015.947319120
CGGTGGG70550.015.901571120
CCGACGG51150.015.364594120
TAGATCC178250.015.279944120
GGACGTG82950.014.9029736
GTCCTAC98500.014.7458311
GTAGGAC101000.014.5591093
AGGACGT92350.014.362485
TAGACGC32150.014.1877454
GACGAGG82650.014.0090885120
CTAGACG18150.013.8887373
TGACGCC66650.013.321618120
TTGACGG21250.013.27360831
GGTACGA15200.013.02688118
AGACCGT30150.012.9374336
Produced by FastQC (version 0.11.2)