FastQCFastQC Report
Mon 11 Dec 2017
WSB-M-ATAC-2-17_TGCTGGGT_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-M-ATAC-2-17_TGCTGGGT_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16292463
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG17800.023.2539141
TAGGACG69200.019.764894
TACGCGT22000.017.723785
ACGCGTA21850.017.2944536
CTACGGT16400.017.1912613
GTCCTAC97500.017.1043741
ATACGCG22300.016.6783264
GTAGGAC103250.016.5003283
TACGGTG18600.016.4482714
TAGACGC30900.015.9191914
GGACGTG84300.015.5112436
CTAGACG19050.015.1150863
CGTACGT17000.014.81969934
CGCGTAT24900.014.6941857
AGGACGT97050.014.6493545
CCGTACG18650.014.15179433
TTATACG19200.014.0597722
TATACGC19750.013.9718873
CCCGATA26350.013.658088
CCTACGG21550.013.63950212
Produced by FastQC (version 0.11.2)