FastQCFastQC Report
Mon 11 Dec 2017
WSB-M-ATAC-2-17_TGCTGGGT_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-M-ATAC-2-17_TGCTGGGT_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16334164
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG56850.023.322641120
CGTATCC146000.017.834164120
TCGCCGG84450.017.121164120
CGGTGGG74500.016.669783120
CGTCTAG19100.016.6518861
GTCCTAC105050.016.5090751
TAGGACG64150.016.3705144
CGCCGTT31450.015.833368120
CCGACGG48750.015.014161120
GTACCGT10700.014.5804836
TAGATCC199800.014.503324120
TGACGCC70900.014.13143120
TACAGTG160550.014.1287065
TACGCCG22150.013.8171155
GACGAGG85150.013.598423120
CTACAGT149750.013.5447444
TAGACCG20100.013.4349915
GGACGTG81300.013.2112796
GTAGGAC99500.013.2081493
AGGACGT91800.013.1393625
Produced by FastQC (version 0.11.2)