FastQCFastQC Report
Mon 11 Dec 2017
WSB-M-ATAC-2-17_TGCTGGGT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-M-ATAC-2-17_TGCTGGGT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16334164
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG18600.024.1916121
TAGGACG72000.017.4138874
GTCCTAC96750.016.5568161
TAGACGC32000.016.3098964
TTGACGG25050.015.56585331
CTAGACG19700.015.2259953
AGGACGT99050.014.8385755
GTAGGAC110650.014.8010713
GGACGTG86150.014.6218416
CGGCTAT29950.013.82049435
CTACGGT18000.013.66406713
TGCACCG28850.012.8921845
TCCTACA146200.012.8854262
GGTACGA17250.012.86676318
GCGTAAA17400.012.75761
TTAGACG19300.012.744083
GTGTAGA95700.012.6635631
CTATACT102200.012.6202954
GACTGTG126700.012.5934047
ACTGCGA39150.012.5641598
Produced by FastQC (version 0.11.2)