FastQCFastQC Report
Mon 11 Dec 2017
WSB-M-ATAC-2-17_TGCTGGGT_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-M-ATAC-2-17_TGCTGGGT_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16347350
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT22200.050.24450794
CGACGTA33250.041.5242892
ACGTATC34650.038.668894
CACCGTA31850.035.66192293
GACGTAT40700.032.75362893
TCGACGT42500.032.4866491
CGCAGTA42050.031.70208491
ACGCCGT33100.031.23297394
GGTCGAC46650.030.03463789
TACGAGT19400.029.79990894
TAGATCG28400.029.21918388
TGGTCGA47800.029.02878288
TCGGTAG22650.028.82769289
GTGGACG41300.028.81945290
TGGACGC43000.027.52190891
ATGTCGG35400.027.09028692
TGTCGGT32600.026.91347793
GGTCGCA51300.026.65049288
GTAGCCG45600.026.54928892
CTCGGTA25400.025.97553888
Produced by FastQC (version 0.11.2)