FastQCFastQC Report
Mon 11 Dec 2017
WSB-M-ATAC-2-17_TGCTGGGT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-M-ATAC-2-17_TGCTGGGT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16347350
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG17850.023.8635441
ATACGCG23750.017.1777174
TAGGACG67400.016.9127354
ACGCGTA23950.016.7817656
TACGCGT22550.016.761555
TAGACGC30350.016.0120334
CGTAATC18100.015.24603622
CTAGACG19700.015.2269173
CGTCTAA18900.014.9193841
GTAGGAC104950.014.7484193
GTCCTAC99200.014.4544331
TTGACGG22900.014.40768631
CCGTACG17750.014.1944333
TACACCG22700.014.0078085
GTACTAG40050.013.93141651
GTGTAGA92650.013.8574471
TATACGC20150.013.3981763
AGGACGT93800.013.3039515
AACTGCG32950.013.2906167
CTACACT92200.013.2745374
Produced by FastQC (version 0.11.2)