FastQCFastQC Report
Mon 11 Dec 2017
WSB-F-ATAC-2-15_TGGATCTG_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-F-ATAC-2-15_TGGATCTG_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14113336
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG18500.019.7865621
CGTATCC102300.019.12082120
TCGCCGG62050.018.856339120
CGCCGTT23750.018.695309120
TCTCGGG49750.017.729185120
CCGACGG35900.017.215012120
TTGACGG19050.017.00815431
CGGTGGG62150.016.508951120
TAGGACG55650.016.4982744
CGTACGT12900.016.27922234
ATACGCG18600.015.8086714
TACGCGT19300.015.5447375
CGAATTG17100.014.73640522
GACGAGG72900.014.568348120
GGATCGT17400.014.4819716
GATCGTT17050.014.42736717
CGCGTAT20050.014.3632577
GTAGGAC85900.014.3209873
CCGTACG14300.014.26596933
GTCCTAC89300.014.1117251
Produced by FastQC (version 0.11.2)