FastQCFastQC Report
Mon 11 Dec 2017
WSB-F-ATAC-2-15_TGGATCTG_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-F-ATAC-2-15_TGGATCTG_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14113336
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT362050.2565304191723346No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG64750.019.6422044
CGTCTAG17900.019.4378871
GGTACGA14200.019.0103718
GTCCTAC83300.017.715941
CGACGAA14050.017.07790823
GTACGAG16750.016.83253319
ACGCGTA19550.016.2631536
TACGGTG13400.016.1151714
ATACGCG19900.015.9777974
TACGCGT20000.015.8976265
GTAGGAC93500.015.5274783
GGACGTG71000.015.4620966
CGTACGT13000.015.22725334
TACGAGG18150.015.20385720
TTGACGG21050.015.10329531
CCGACGA16600.014.8161222
AGACCGT23200.014.7388136
TTGACGA40750.014.72000760
AGGACGT85850.014.6745715
CCGTACG13500.014.66317733
Produced by FastQC (version 0.11.2)