FastQCFastQC Report
Mon 11 Dec 2017
WSB-F-ATAC-2-15_TGGATCTG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-F-ATAC-2-15_TGGATCTG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14109506
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT357960.2537012989682275No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG16000.023.245711
CTAGACG17650.020.3947163
CTACGGT16650.020.17524113
TACGGTG17550.018.45702214
TAGGACG65350.018.1773554
GGTACGA13350.017.07622518
TAGACGC28100.016.6532654
CCGTACG12650.016.5981399
TAATCGA15800.016.3277221
ACGTACG12600.016.18685737
GTAGGAC94500.016.0620083
TTGACGG19950.015.93623131
GTCCTAC88500.015.7259121
CGACTTG42100.015.69782264
CGTACGT12200.015.2421710
GGACGTG77650.015.0654186
GACGACT43800.015.06480662
GTACGAG16000.014.99850819
TATCGAA17750.014.86970448
CGTACGG13050.014.70940838
Produced by FastQC (version 0.11.2)