FastQCFastQC Report
Mon 11 Dec 2017
WSB-F-ATAC-2-15_TGGATCTG_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-F-ATAC-2-15_TGGATCTG_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14095484
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCC109300.021.463516120
CGTAATC15750.020.57153522
CGTACGT14800.019.05814434
TAGGACG64200.018.7871444
CCGACGG36350.018.321577120
CCGTACG15850.017.79656233
TCTCGGG51450.017.49243120
TCGCCGG63850.017.478186120
CGGTGGG60700.017.001375120
CGTCTAG17650.016.6590841
GACGAGG68850.015.686023120
TAGATCC136050.015.611656120
AGGACGT84200.015.321185
ACGTACG14200.015.211437
CGCCGTT24100.015.186478120
GTCCTAC88150.015.1803671
GGACGTG76900.014.9805736
CGTACGG14600.014.38287138
TTGACGA42150.014.37638260
GGACGTA23800.014.36956819
Produced by FastQC (version 0.11.2)