FastQCFastQC Report
Mon 11 Dec 2017
WSB-F-ATAC-2-15_TGGATCTG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-F-ATAC-2-15_TGGATCTG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14095484
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT355500.25220843782306446No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG18850.020.049641
TACGGTG15250.017.30699514
TTGACGG21150.016.73373431
TAGGACG59650.016.696224
CTAGACG18400.015.9771313
CTACGGT16700.015.80429313
TAGTCGA14500.014.8953915
CGTAATC16150.014.86036822
TAGACGC31100.014.8542554
GGTACGA12650.014.70079618
TACGCCG12250.014.6927355
CGACGAA14450.014.53251423
ATCATCG19100.014.44698530
AACTGCG26600.014.4336877
GCGAATT16250.014.39954419
TACACCG20450.014.3754095
GTCCTAC89800.014.2960091
AGGACGT81650.014.1813835
CGTCTAA18250.014.13458251
GACCGTT18700.014.1152957
Produced by FastQC (version 0.11.2)