FastQCFastQC Report
Mon 11 Dec 2017
WSB-F-ATAC-2-15_TGGATCTG_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-F-ATAC-2-15_TGGATCTG_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14064238
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG20150.020.2510831
CGTATCC103400.019.08968120
TCGCCGG62450.018.44557120
TCTCGGG45700.017.723148120
TTGACGG20100.017.31718331
TAGGACG59550.017.1302834
CGCCGTT21900.016.985209120
CCGACGG34900.016.675144120
GTCCTAC89900.015.8866261
GACGAGG68550.015.491344120
GCGAATT16850.014.95622819
TTGACGA36050.014.31273660
CGGTGGG64000.014.249083120
GTAGGAC90950.014.1856793
ACGGCTA25400.014.17569334
TACCGCG16200.014.0731689
CTACGGT15350.014.07125613
TGACGCC51350.014.02052120
CGGCTAT29500.013.83109435
CGTACGT13950.013.76583534
Produced by FastQC (version 0.11.2)