FastQCFastQC Report
Mon 11 Dec 2017
WSB-F-ATAC-2-15_TGGATCTG_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-F-ATAC-2-15_TGGATCTG_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14064238
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT352050.2503157298674838No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG17100.021.0498091
TTGACGG20200.017.22308331
TAGGACG59250.016.9096644
CTAGACG19250.016.8294493
TAGTCGA12600.016.1901455
CTACGGT15800.015.94598113
TAGACGC28900.015.7769344
CGTACGT12150.015.30593510
GCGAATT16250.015.13519219
CGTCGTG17900.015.07973828
CGGCTAT26200.014.88159435
TAGACCG16700.014.7290334
CCGTACG13850.014.7268489
CTATACT91650.014.5975274
TACGGTG16850.014.59656414
GTAGGAC90650.014.5600133
GTAGCGT19050.014.48480324
GTCCTAC86450.014.4341541
TTGACGA40600.014.33111560
ATACGCG20100.014.3268954
Produced by FastQC (version 0.11.2)