FastQCFastQC Report
Mon 11 Dec 2017
WSB-F-ATAC-2-15_TGGATCTG_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-F-ATAC-2-15_TGGATCTG_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14025545
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG50100.022.512846120
CCGACGG34850.020.485872120
TCGCCGG59500.020.266994120
CGTATCC104300.018.751825120
CGCCGTT22850.018.116453120
CGTACGT13300.018.049234
GGTACGA14400.017.50003418
TAGGACG60000.017.0037924
CGTAATC16700.016.88740322
CCGTACG14750.016.68204133
GTCCTAC86100.015.8923281
TAGATCC138750.015.393142120
GACGAGG71600.015.33375120
CGGTGGG65800.015.044172120
CGTCTAG18000.014.6701991
GGACGTG71800.014.6264346
GTATAGG45600.014.6087811
GTAGGAC91450.014.5688453
TATCGAA16100.014.53273848
TTGACGA38300.014.25605460
Produced by FastQC (version 0.11.2)