FastQCFastQC Report
Mon 11 Dec 2017
WSB-F-ATAC-2-15_TGGATCTG_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-F-ATAC-2-15_TGGATCTG_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14025545
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT355620.2535516445172006No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG17550.022.2199121
TACGGTG17050.020.75978714
CTACGGT18050.019.9397513
CTAGACG19900.019.8974293
TAGGACG59750.019.8805664
TAGACGC30100.018.1374534
TTGACGG19100.017.90140731
TCCGCAA20850.017.26199233
TTGACGA39600.016.96545260
CGTAATC15450.016.69616322
CGTACGT12450.016.38167834
GACGACT39200.016.37368862
CGACTTG39050.016.15504364
CCTACGG22900.015.97973112
TACGCGT20400.015.8805315
GTAGGAC91350.015.8275823
AGGACGT77800.015.8079565
GTCCTAC87800.015.7842351
GGACGTG70200.015.5539386
CGGCTAT27000.015.55188635
Produced by FastQC (version 0.11.2)