FastQCFastQC Report
Mon 11 Dec 2017
WSB-F-ATAC-2-15_TGGATCTG_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-F-ATAC-2-15_TGGATCTG_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14056072
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG51050.019.390917120
CGTATCC114950.018.684618120
GGTACGA13800.017.39157718
TAGGACG60500.017.2608914
CCGACGG34800.017.06735120
CGGTGGG60950.016.93031120
TCGCCGG61000.016.916431120
GTCCTAC89100.016.7726541
GCGAATT16950.016.63766519
CGTAATC16450.016.4140422
CGTCTAG17100.015.4431791
GACGAGG72900.014.978006120
GATGCGA19600.014.69405616
CGCCGTT23150.014.512666120
GTACGAG16150.014.48959819
TAGATCC151250.014.477969120
GGACGTG69850.014.2608226
TGCGAAT19450.014.19045418
GGTCGGT30800.014.02504511
AGGACGT81200.013.8210485
Produced by FastQC (version 0.11.2)