FastQCFastQC Report
Mon 11 Dec 2017
WSB-F-ATAC-2-15_TGGATCTG_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-F-ATAC-2-15_TGGATCTG_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14056072
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT359690.25589652642644406No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG16100.021.98381
CTAGACG16200.017.0338693
TAGGACG61300.016.9299684
TAGACCG18500.016.21325
GTCCTAC86050.015.6161441
TTGACGA37650.015.61312660
TAGACGC28200.015.3162994
GCGAATT17650.015.29357219
TACGCCG13500.015.1083015
GATGCGA19900.015.07209116
CGTCAGA24400.014.9974241
ACTGCGA31400.014.9002038
TTGACGG18650.014.79533431
GTAGGAC92450.014.664663
ATGTCGA17800.014.49065518
ATACGCG19050.014.4854954
ACGCGTA19250.014.3336196
TATCGAA16850.014.24097948
CGTCTAA17700.014.2348431
TGCGAAT19600.014.078011518
Produced by FastQC (version 0.11.2)