FastQCFastQC Report
Mon 11 Dec 2017
WSB-F-ATAC-2-15_TGGATCTG_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-F-ATAC-2-15_TGGATCTG_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14056315
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCC115200.019.633589120
TAGGACG64100.018.7247984
TCGCCGG61000.018.686783120
CCGACGG34350.018.51356120
TCTCGGG46950.017.761923120
CGCCGTT23100.017.66069120
CGTCTAG19950.017.4473841
CGGTGGG64100.016.285534120
TTGACGG17150.016.09505831
CGCGTAT19150.015.3527427
GACGAGG69400.015.301168120
GCGAATT16100.015.27992719
TGACGCC54050.015.206722120
ATACGCG19200.015.0032464
GGACGTG75800.014.9618476
GTCCTAC89850.014.8947081
AGGACGT85550.014.5209675
TAGATCC162850.014.478233120
CGGCTAT23400.014.36039535
TACGCGT20100.014.3314585
Produced by FastQC (version 0.11.2)