FastQCFastQC Report
Mon 11 Dec 2017
WSB-F-ATAC-2-15_TGGATCTG_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-F-ATAC-2-15_TGGATCTG_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14056315
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT356720.2537791732754993No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG18550.026.519051
TTGACGG19600.021.11802131
CTACGGT15400.017.91851213
TAGGACG61950.017.817344
CGTACGG14500.017.7896938
TAGACGC29450.017.3141024
TACGGTG16050.017.1928414
GTCCTAC86300.017.1701531
ACGTACG15250.016.91532937
GTAGGAC95050.016.0305333
TACGCCG12400.015.9645635
CGGCTAT25800.015.81075135
TTGACGA40400.015.590013560
CTAGACG20050.015.5580573
GACGACT40250.015.20161962
ACGGCTA25900.015.05475934
ATACGCG18200.014.8322664
AACTGCG28250.014.6510137
CGACTTG39300.014.52299164
CGACGAA14550.014.429399523
Produced by FastQC (version 0.11.2)