FastQCFastQC Report
Mon 11 Dec 2017
WSB-F-ATAC-2-15_TGGATCTG_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-F-ATAC-2-15_TGGATCTG_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14068474
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT16100.051.54821494
CGACGTA26650.048.75462392
TCGACGT27950.046.97393491
GGTCGAC32200.040.35208589
ACGTATC30100.040.00243894
GTCGACG33550.039.53880390
TGTCGGT22250.039.13448793
TGGTCGA34600.037.9477188
GTGATCG19300.036.30457791
ACGCCGT23900.035.57884294
ATGTCGG26450.034.72064692
GACGTAT36300.033.7322793
TACGAGT15700.033.36343894
GTCGATC21650.033.306492
TAGATCG20250.033.25940788
TGATCGC20950.033.1204292
ATCGCCG19800.032.29552594
CACCGTA26000.032.1818893
GTCGGTG28350.030.71401694
GTGTCGA23300.030.3639990
Produced by FastQC (version 0.11.2)