FastQCFastQC Report
Mon 11 Dec 2017
WSB-F-ATAC-2-15_TGGATCTG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWSB-F-ATAC-2-15_TGGATCTG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14068474
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT350170.2489040389170851No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG15800.023.5413441
ATACGCG19850.017.5294064
ACGCGTA20650.017.139186
GTCCTAC87400.017.0230311
TACGCGT20500.016.9735975
TTGACGG18600.016.77089131
TAGGACG59200.016.720994
CTAGACG17400.016.2047423
CGCGTAT22800.015.5233157
CGTAATC15950.015.044167522
CGTACGT13250.014.940454534
GTAGGAC90950.014.90731053
TTGACGA41500.014.5990760
TAGTCGA13400.014.3266595
GGTACGA14250.014.31368418
CCGTACG13550.014.16695533
CGACTTG39900.014.14462364
TATACGC17400.014.1360523
TTATACG18750.014.078232
GGACGTG70000.014.0541276
Produced by FastQC (version 0.11.2)