FastQCFastQC Report
Mon 11 Dec 2017
PWK-M-frozen-3-library_ACCACTGT_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-M-frozen-3-library_ACCACTGT_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10571764
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG17550.036.5855941
TTGACGG20900.027.56038731
CGGTGGG42550.025.101122120
TAGGACG44600.025.025434
TCTCGGG33600.024.644073120
CGGCTAT26050.024.41463935
TCGCCGG51050.023.625063120
CGCCGTT17750.023.325094120
GTCCTAC78500.022.1682721
CGTATCC96900.021.549051120
GGTACGA15450.021.35795618
GGACGTG52000.021.114436
TGACGGC27300.021.09934432
CCGACGG26600.020.527328120
CTCGAAT14950.020.4670112
ACGGCTA27900.020.21547934
TAGTGCG14000.020.1432828
CTACAGT92500.020.0456544
CGCAATT13500.019.99975649
TACAGTG96550.019.638375
Produced by FastQC (version 0.11.2)