FastQCFastQC Report
Mon 11 Dec 2017
PWK-M-frozen-3-library_ACCACTGT_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-M-frozen-3-library_ACCACTGT_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10571764
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCACTGTATCTCGTATGCCGT114010.10784387544027657No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG15600.036.147721
TAGGACG45350.025.7964324
TTGACGG21600.023.6056631
GGTACGA15250.022.81537618
GGACGTG51550.022.3440886
TATGGCG58050.021.59779516
GTACGAG16450.021.15103119
GTTCGCA14800.021.0778089
TGGTACG16850.021.00494817
TACGTAC7750.020.8996851
TACGAGG17000.020.81951120
TACGGTG13050.020.68497814
CTACGGT13050.020.68497813
TGCGACC12500.020.63543110
GTATAGG41750.020.5474191
TGACGGC26150.020.18654332
GGTCGGT29000.019.85767211
TCCGCAA13950.019.7803833
CGTACAC11050.019.5455883
ACGATCA17200.019.53037529
Produced by FastQC (version 0.11.2)