FastQCFastQC Report
Mon 11 Dec 2017
PWK-M-frozen-3-library_ACCACTGT_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-M-frozen-3-library_ACCACTGT_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10516112
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG15550.030.8716321
TCTCGGG28300.026.078186120
TCGCCGG51050.023.976906120
TTGACGG19150.023.18654631
TATTACG8350.022.99320236
CGGTGGG40550.022.491138120
TAGGACG44300.021.9437714
CGTGATT35150.021.33840428
CGTATCC103350.021.07438120
CGCCGTT17700.021.01731120
CCGACGG27750.020.973331120
CGTAATC15350.020.71588322
ATTACGC9300.020.64433737
CCGTGAT35550.020.42316427
CTCGAAT14200.019.85836212
TAGTGCG14300.019.72146228
GTCCTAA54650.019.654541
TACAGTG96950.019.618235
GGACGTG50250.019.4619086
GCGTTTA12800.019.2185335
Produced by FastQC (version 0.11.2)